|
KMID : 1123920080220020415
|
|
Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 2 p.415 ~ p.421
|
|
|
Euphorbiae Humifusae Inhibits Egr-1, and COX-2 Activity Stimulated by Phorbol 12-myristate-13-acetate
|
|
Kim Cheng-Yun
Park Sang-Eun
Kim Won-Ill
Park Dong-Il
Kim Ki-Young
Kim Nam-Deuk
Hong Sang-Hoon
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Pro-inflammatory mediators, such as prostaglandin (PGE2), nitric oxide (NO), and cyclooxygenases-2 (COX-2), play pivotal roles in normal as well as transformed cells. Previous studies have shown that Euphorbiae humifusae Wind exhibits anti-proliferative and antioxidant activities. However, the it’s anti-inflammatory properties are unclear. In this study, we examine the effects of water extract of E. humifusae (WEEH) on the expression of COX-2 and the production of in human lymphatic U937 cells. Treatment of phorbol 12-myristate-13-acetate (PMA) significantly induced COX-2 expression and production in U937 cells. However, pretreatment WEEH markedly inhibited the PMA-induced COX-2 expression and production in a dose-dependent manner. Moreover, WEEH prevented the elevated early growth response gene-1 (Egr-1) expression and nuclear factor-kappaB () nuclear translocation stimulated by PMA treatment. Taken together, the present data indicate that WEEH exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the and Egr-1 signaling pathway.
|
|
|
KEYWORD
|
|
|
Euphorbiae humifusae, cyclooxygenases-2, prostaglandin E2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|